Genetic and morphological distinctness of three cyprinid species from Isle Royale and Voyageurs National Parks
On the basis of morphological measurements, previous investigators concluded that three cyprinid species, fathead minnow (Pimephales promelas), blacknose shiner (Notropis heterolepis), and pearl dace (Margariscus margarita), from Harvey Lake in Isle Royale National Park were unique enough to warrant subspecific status. Other investigators, however, have questioned these designations and concluded the Harvey Lake fish do not merit such designation. WHAT: Samples of the three species will be collected from Harvey Lake and other lakes on Isle Royale, from mainland sites along the north shore of Lake Superior in Minnesota, from lakes and ponds in Voyageurs National Park, and from waters in the upper Mississippi River drainage. This will result in our having samples from three major drainages: Great Lakes, Hudson Bay, and Mississippi. Genetic analyses will be conducted using the microsatellite DNA analysis technique. Allelic type and frequency at each locus will be statistically analyzed to determine if there is significant population structuring present. The results of these analyses will be analyzed statistically using Wrights Fst statistics. Morphological measurements will be made from fish from the same sites as those for which genetic analyses have been made. The resulting data will be summarized and statistically analyzed using standard PC software such as Lotus 1-2-3 and SYSTAT. Keywords:Aquatic,Ecosystem,Fisheries Mgmt.,Population,Resources, Small lakes,Status,Trend.