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Molecular genetic analysis of bacterial sources in the Clinton River watershed

This study investigated whether direct sequencing of a bacterial   gene could be utilized for detecting genetic differences between   bacterial strains for microbial source tracking. A 525-nucleotide   segment of the gene for B-glucuronidase (uidA) was sequenced in 941   Escherichia coli isolates from the Clinton River-Lake St. Clair   watershed, 182 E. coli isolates from human and animal feces, and 34   E. coli isolates from a combined sewer. Environmental isolates   exhibited 114 alleles in 11 groups on a genetic tree. Frequency of   strains from different genetic groups differed significantly.     This project demonstrates differences in the frequency of different   E. coli strains at different locations in a watershed, and provide   a "proof in principle" that sequence-based data can be used for   microbial source tracking.    (microbial source tracking); (MST); (bacterial source tracking);   (BST)

Status
In progress
Type
Project
Start Date
End Date
Researchers
Jeffrey L. RamPrincipal Investigator
Associated with 3 projects
Agencies

Funding 1 projects for a total of $50,000.00

Funding 2 projects for a total of $60.00
Scope of Study
Field Investigation
Laboratory Investigation
Scale of Phenomena
Cellular
Lake Basin Connecting Channels
Lake St. Clair
State Province
Michigan
Resource Being Monitored
Human Health
Annex Numbers
Pollution from Contaminated Groundwater
Pollution from Non-Point Sources
Remedial Action Plans and Lakewide Management Plans
General
Annex
  • Annex Numbers
    Annex Numbers
    Pollution from Contaminated Groundwater
    Pollution from Non-Point Sources
    Remedial Action Plans and Lakewide Management Plans
  • Annex 17

The Great Lakes - St. Lawrence Research Inventory is an
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up-to-date information about research projects in the
Great Lakes - St. Lawrence Region.