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Isolation of viral haemorrhagic septicaemia virus from muskellunge, Esox masquinongy (Mitchill), in Lake St Clair

Viral haemorrhagic septicaemia virus (VHSV) was isolated from   muskellunge, Esox masquinongy (Mitchill), caught from the NW portion   of Lake St Clair, Michigan, USA in 2  3. Affected fish exhibited   congestion of internal organs; the inner wall of the swim bladder   was thickened and contained numerous budding, fluid-filled vesicles.   A virus was isolated using fish cell lines inoculated with a   homogenate of kidney and spleen tissues from affected fish. Focal   areas of cell rounding and granulation appeared as early as 24 h   post-inoculation and expanded rapidly to destroy the entire cell  sheet by 96 h. Electron microscopy revealed virions that were 17 -  18  nm in length by 6 -7  nm in width having a bullet-shaped   morphology typical of rhabdoviruses. The virus was confirmed as VHSV   by reverse transcriptase-polymerase chain reaction.  Sequence analysis of the entire nucleoprotein and glycoprotein genes   revealed the virus was a member of the North American genotype of   VHSV; however, the isolate was sufficiently distinct to be considered  a separate sublineage, suggesting its origin may have been from   marine species inhabiting the eastern coastal areas of the USA or   Canada.    (VHS); (VHSv); (viral hemorrhagic septicemia); (viral haemorrhagic   septicaemia); (viral hemmorrhagic septicemia)

Status
In progress
Type
Project
Start Date
End Date
Researchers
James WintonPrincipal Investigator
Associated with 3 projects
Mohammed FaisalPrincipal Investigator
Associated with 2 projects
Gary WhelanResearcher
Associated with 3 projects
Agencies
U.S. Geological Survey $ 290,000.00USDEstimates

Funding 13 projects for a total of $538,321.00
Scope of Study
Field Investigation
Laboratory Investigation
Scale of Phenomena
Cellular
Organism
Impact of Pollutants
Exotic Species
Resource Management
Fisheries
Lake Basin Connecting Channels
Lake St. Clair
State Province
Michigan
Resource Being Monitored
Fisheries
Beneficial Use Impairment Assessments
Fish Tumours or Other Deformities
Loss of Fish and Wildlife Habitat
Tainting of Fish and Wildlife Populations
Biology And Life History
Parasites and Diseases
Annex Numbers
Research & Development
Annex 17
Impact of water quality and AIS on fish and wildlife populations and habitats
General
Monitoring
Annex
  • Annex Numbers
    Annex Numbers
    Research & Development
  • Annex 17
    Annex 17
    Impact of water quality and AIS on fish and wildlife populations and habitats

The Great Lakes - St. Lawrence Research Inventory is an
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Great Lakes - St. Lawrence Region.