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Differentiation of fecal Escherichia coli from poultry and free-living birds by (GTG)5-PCR genomic fingerprinting

Determination of the non-point sources of fecal pollution is   essential for the assessment of potential public health risk and   development of appropriate management practices for prevention of   further contamination. Repetitive extragenic palindromic-PCR coupled   with (GTG)5 primer [(GTG)5-PCR] was performed on 573 Escherichia coli  isolates obtained from the feces of poultry (chicken, duck and   turkey) and free-living (Canada goose, hawk, magpie, seagull and   songbird) birds to evaluate the efficacy of (GTG)5-PCR genomic   fingerprinting in the prediction of the correct source of fecal   pollution. A discriminant analysis with the jack-knife algorithm of   (GTG)5-PCR DNA fingerprints revealed that 95%, 94.1%, 93.2%, 84.6%,   79.7%, 76.7%, 75.3% and 7 .7% of magpie, hawk, turkey, seagull,   Canada goose, chicken, duck and songbird fecal E. coli isolates   classified into the correct host source, respectively.     The results of this study indicate that (GTG)5-PCR can be considered   to be a complementary molecular tool for the rapid determination of   E. coli isolates identity and tracking the non-point sources of   fecal pollution.    (microbial source tracking); (MST); (bacterial source tracking);   (BST)

In progress
Start Date
End Date
Scope of Study
Field Investigation
Laboratory Investigation
Resource Being Monitored
Human Health
Annex Numbers
Pollution from Non-Point Sources

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