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Identifying Host Sources of Fecal Pollution: Diversity of Escherichia coli in Confined Dairy and Swine Production Systems

Repetitive extragenic palindromic PCR fingerprinting of Escherichia   coli is one microbial source tracking approach for identifying the   host source origin of fecal pollution in aquatic systems. The   construction of robust known-source libraries is expensive and   requires an informed sampling strategy. In many types of farming  systems, waste is stored for several months before being released   into the environment.     In this study we analyzed, by means of repetitive extragenic   palindromic PCR using the enterobacterial repetitive intergenic   consensus primers and comparative analysis using the Bionumerics   software, collections of E. coli obtained from a dairy farm and from   a swine farm, both of which stored their waste as a slurry in   holding tanks. In all fecal samples, obtained from either barns or   holding tanks, the diversity of the E. coli populations was   underrepresented by collections of 5   isolates. In both the dairy   and the swine farms, the diversity of the E. coli community was   greater in the manure holding tank than in the barn, when they were   sampled on the same date. In both farms, a comparison of stored   manure samples collected several months apart suggested  that the community composition changed substantially in terms of the   detected number, absolute identity, and relative abundance of   genotypes.     Comparison of E. coli populations obtained from 1  different   locations in either holding tank suggested that spatial variability   in the E. coli community should be accounted for when  sampling. Overall, the diversity in E. coli populations in manure   slurry storage facilities is significant and likely is problematic   with respect to library construction for microbial source tracking   applications.    (microbial source tracking); (MST); (bacterial source tracking);   (BST)

Status
In progress
Type
Project
Start Date
End Date
Scope of Study
Field Investigation
Laboratory Investigation
State Province
Ontario
Annex Numbers
Pollution from Contaminated Groundwater
Pollution from Non-Point Sources
Remedial Action Plans and Lakewide Management Plans

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