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A Preliminary Study of Escherichia coli in Three Canadian Watersheds with Respect to their Phylogenetic Group, Virulence and antimicrobial Resistance GeneComposition

Fifty isolates of Escherichia coli were obtained from each of three   Canadian agricultural watersheds: the Oldman River in Alberta, the   South Nation watershed in Ontario and the Bras d'Henri/Fourchette  River in Quebec. Water samples were collected biweekly in 2  5 from   2  sites across the three watersheds. E. coli isolates were   recovered by membrane filtration and incubation at 44.5 C. For each  watershed, 25 isolates were chosen over the sampling period from   each of the most and least fecally contaminated sites. Isolated   Escherichia coli colonies were selected randomly from agar plates,   grown under standard conditions, and their taxonomic identification   confirmed. Their DNA was extracted and hybridized to a microarray   carrying probes for 189 virulence-related genes and 3  antimicrobial  resistance genes. The microarray also carried probes for the three   markers used for phylogenetic group determination (chuA, yjaA and   TSpe4.C2). The percentage of Escherichia coli with a pattern of  virulence genes indicating a pathotype was fairly consistent   (between 24% and 33%) while the percentage of isolates with at least   one antimicrobial resistance gene showed more variation (between  6% and 22%). Further studies are being considered to explore the   relationships between source of pollution, virulence and   antimicrobial resistance genes in waterborne Escherichia coli.    (microbial source tracking); (MST); (bacterial source tracking);   (BST)

Status
In progress
Type
Project
Start Date
End Date
Scope of Study
Field Investigation
Laboratory Investigation
State Province
Ontario
Quebec
Resource Being Monitored
Human Health
Beneficial Use Impairment Assessments
Beach Closings
Restrictions on Drinking Water Consumption or Taste and Odour Problems
Annex Numbers
Pollution from Contaminated Groundwater
Pollution from Non-Point Sources
Remedial Action Plans and Lakewide Management Plans
Monitoring
Annex
  • Annex Numbers
    Annex Numbers
    Pollution from Contaminated Groundwater
    Pollution from Non-Point Sources
    Remedial Action Plans and Lakewide Management Plans
  • Annex 17

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