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DEVELOPMENT OF AN IMPROVED MEDIUM FOR PRIMARY ISOLATION OF FLAVOBACTERIUM PSYCHROPHILUM, CAUSE OF BACTERIAL COLDWATER DISEASE

Research on the Flavobacterial pathogens of fishes e.g. F.   psychrophilum has been hampered by their inherent difficulties of   primary isolation from fish, growth, and storage and recovery.   Fungal overgrowth on primary isolation plates is also a major   problem. There is a need for an improved isolation and growth   medium, especially for (early) primary isolation from carrier,   asymptomatic fish. Recently, we formulated a medium to recover F.   psychrophilum isolates from long-duration frozen storage. We   recovered 93+ % of about 15  isolates; this compared with   % in a   previous attempt with the medium that most diagnosticians and   researchers have historically employed. Arguably, recovery of F.   psychrophilum from frozen storage is more challenging than primary   isolation from diseased fish. We propose to develop this medium, to   include contaminant deterrents, for use for primary isolation of F.   psychrophilum.  The new medium (currently referred to as '#2') will be developed and   tested at the National Fish Health Research Laboratory. A battery of   media will be used to evaluate the performance of #2 in the   laboratory: #2, cytophaga medium and EAOA+FBS. Individual   ingredients of #2 will be evaluated to maximize the medium's   performance and various serum supplements will be tested. Field   testing will be done on samples from selected fish hatcheries in the   US and Canada experiencing overt disease, moribund fish will be   assayed to compare the performance of control media to the improved   media (with and without antifungal supplementation) for primary   isolation of F. psychrophilum. Isolated bacteria will be   characterized. Data will include bacterial growth performances of   the new vs. currently used media, pathogen prevalence within and   among hosts, and percent recoveries using the various media.

Status
In progress
Type
Project
Start Date
End Date
Scope of Study
Field Investigation
Laboratory Investigation
Scale of Phenomena
Organism
Population
Resource Management
Fisheries
Lake Basin Connecting Channels
Lake Erie
Lake Michigan
Lake Ontario
State Province
New York
Wisconsin
Annex Numbers
Research & Development

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up-to-date information about research projects in the
Great Lakes - St. Lawrence Region.