Skip to main content
hyi
Banner
banner
bn

Rapid and Quantitative Detection of Helicobacter Pylori and E. Coli O157 in Well Water Using a Nano-Wired Biosensor and QPCR

Objective:  The hypothesis of this project is that a disposable biosensor and   QPCR can be combined seamlessly to develop a unique biosensor-QPCR   as a tool for near real-time determination of contaminant occurrence   in drinking water.   The specific objectives are: (1) Develop a protocol for processing   water samples for the biosensor and QPCR; (2) Assess the performance   of the biosensor and QPCR for sensitivity, specificity, recovery,   and false positives/negatives of detection and enumeration for E.   coli O157:H7 and H. pylori in groundwater samples from the field;   (3) Develop a method for detecting and enumerating E. coli O157:H7   and H. pylori by QPCR using bacteria isolated and screened by the   biosensor system; and (4) Validate a method for testing viability of   E. coli O157:H7.    (biosensor-QPCR technique); (drinking water); (restrictions on   drinking water); (pathogens); (RFA); (microorganisms); (bacteria);   (nanotechnology); (biotechnology); (pathogens); (Helicobacter   pylori); (E. Coli); (Escherichia coli)

Status
In progress
Type
Project
Start Date
End Date
Scope of Study
Field Investigation
Laboratory Investigation
Scale of Phenomena
Biochemical
Physical/Chemical
Resource Being Monitored
Human Health
Beneficial Use Impairment Assessments
Restrictions on Drinking Water Consumption or Taste and Odour Problems
Annex Numbers
Pollution from Contaminated Groundwater
Research & Development
Annex 17
Control technologies for treatment of municipal and industrial effluents/emissions
General
Monitoring
Annex
  • Annex Numbers
    Annex Numbers
    Pollution from Contaminated Groundwater
    Research & Development
  • Annex 17
    Annex 17
    Control technologies for treatment of municipal and industrial effluents/emissions

The Great Lakes - St. Lawrence Research Inventory is an
interactive, Internet-based, searchable database created as a tool to collect and disseminate
up-to-date information about research projects in the
Great Lakes - St. Lawrence Region.