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A comparative study of culture-independent, library-independent genotypic methods of fecal source tracking

Culture-independent fecal source tracking methods have many   potential advantages over library-dependent, isolate-culture   methods, but they have been subjected to limited testing. The  purpose of this study was to compare culture-independent, library-  independent methods of fecal source tracking. Five laboratories   analysed identical sets of aqueous samples that contained one or  more of the following sources: sewage, human feces, dog feces,   cattle feces and gull feces. Two investigators used methods based on   PCR amplification of Bacteroidetes marker genes and both  successfully discriminated between samples that did or did not   contain human fecal material. One of these investigators was also   able to identify the remaining sources, except for gull, with a low   rate of false positives. A method based on E. coli toxin genes   successfully identified samples containing sewage and cattle feces,   but missed some samples with human feces because of low marker  prevalence in individual human fecal samples. Researchers who used   community terminal restriction fragment length polymorphism (T-RFLP)   were limited by the amount of DNA recovered from samples, but they   correctly identified human and cattle fecal contamination when   sufficient DNA was obtained.     (bacteroides); (culture-independent); (fecal source tracking);   (microbial source tracking); (MST); (bacterial source tracking);   (BST); (restriction fragment length polymorphism); (polymerase chain   reaction); (PCR); (RFLP)

Status
In progress
Type
Project
Start Date
End Date
Scope of Study
Laboratory Investigation
Literature / Existing Data
Annex Numbers
Pollution from Contaminated Groundwater
Pollution from Non-Point Sources

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