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Survival of the North American strain of viral hemorrhagic septicemiavirus (VHSV) in filtered seawater and seawater containing ovarianfluid, crude oil and serum-enriched culture medium

The North American strain of viral hemorrhagic septicemia virus (NA-  VHSV) could be recovered for up to 4  h in natural filtered seawater   (27 ppt) with a 5 % loss of infectivity after approximately 1  h at   15°C. Addition of 1  ppb North Slope crude oil to the seawater had   no effect on virus survival. However, when various concentrations of  teleost ovarian fluid were added to seawater, virus could be  recovered after 72 h at  . 1% ovarian fluid and after 96 h at 1. %.   When cell culture medium supplemented with 1 % fetal bovine serum   was added to the seawater, 1  % of the virus could be recovered for   the first 15 d and 6 % of the virus remained after 36 d. These   findings quantify NA-VHSV infectivity in natural seawater and   demonstrate that ovarian fluid, which occurs naturally during   spawning events, significantly prolongs the survival and infectivity   of the virus. The extended stabilization of virus in culture medium   supplemented with serum allows for low titer field samples to be   collected and transported in an unfrozen state without significant  loss of virus titer.    (VHS); (VHSv); (viral hemorrhagic septicemia); (viral haemorrhagic   septicaemia); (viral hemmorrhagic septicemia)

Status
In progress
Type
Project
Start Date
End Date
Researchers
Richard KocanPrincipal Investigator
Associated with 1 projects
Paul HershbergerResearcher
Associated with 2 projects
Nancy ElderResearcher
Associated with 1 projects
Agencies
Exxon Valdez Trustee Council $ 0.00USDEstimates

Funding 1 projects for a total of $0.00

Funding 1 projects for a total of $0.00
Scope of Study
Field Investigation
Laboratory Investigation
Impact of Pollutants
Exotic Species
Resource Management
Fisheries
Resource Being Monitored
Fisheries
Beneficial Use Impairment Assessments
Fish Tumours or Other Deformities
Loss of Fish and Wildlife Habitat
Tainting of Fish and Wildlife Populations
Biology And Life History
Parasites and Diseases
Annex Numbers
Research & Development
Review of Pollution from Shipping Sources
Annex 17
Impact of water quality and AIS on fish and wildlife populations and habitats
General
Monitoring
Annex
  • Annex Numbers
    Annex Numbers
    Research & Development
    Review of Pollution from Shipping Sources
  • Annex 17
    Annex 17
    Impact of water quality and AIS on fish and wildlife populations and habitats

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