Skip to main content
hyi
Banner
banner
bn

Molecular Assessment of the Potential for in situ PCB Bioremediation

Objectives:  1) To determine the efficacy of a Polymerase Chain Reaction (PCR) assay in determining the genetic potential for in situ bioremediation of polychlorinated biphenyl (PCB)-contaminated sediments in the Great Lakes. 2)  To investigate the capacity of sediments having different molecular genetic profiles in their ability to degrade complex PCB mixtures. Abstract:  PCBs are a family of xenobiotic compounds that are ubiquitous and persistent pollutants. Thus, PCBs are a common target of sediment remediation efforts. Microbial degradation of PCBs offers an environmentally sound and economically favorable alternative to conventional means of remedaition such as dredging. This project describes the development of a PCR-based assay to determine the potential for PCB bioremediation by the resident microbial consortium in contaminated sediments. By PCR and RT-PCR of nucleic acids extracted from Lake Erie sediments, we can amplify the bphA1 gene encoding the large subunit of biphenyl diooxygenase. Since work in other labs have shown that the BphA1 protein dictates (in part) PCB congener specificity, our assay may prove to be a useful screen for endemic catabolic activities for PCB mixtures in contaminated sediments.

Status
In progress
Type
Project
Start Date
End Date
Scope of Study
Laboratory Investigation
Scale of Phenomena
Physical/Chemical
Impact of Pollutants
Toxic Organics
Lake Basin Connecting Channels
Lake Erie
State Province
Ohio
Annex Numbers
Contaminated Sediment
Hazardous Polluting Substances - lists
Persistent Toxic Substances
Research & Development
Specific Objectives for toxic substances and effluents
Surveillance and Monitoring
Annex 17
Relationship of contaminated sediments on ecosystem health
General
Annex
  • Annex Numbers
    Annex Numbers
    Contaminated Sediment
    Hazardous Polluting Substances - lists
    Persistent Toxic Substances
    Research & Development
    Specific Objectives for toxic substances and effluents
    Surveillance and Monitoring
  • Annex 17
    Annex 17
    Relationship of contaminated sediments on ecosystem health

The Great Lakes - St. Lawrence Research Inventory is an
interactive, Internet-based, searchable database created as a tool to collect and disseminate
up-to-date information about research projects in the
Great Lakes - St. Lawrence Region.