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Combining an optical strip-assay biosensor with ribotyping for bacterial source tracking of Enterococcus faecalis in the Lower Hudson River basin

Project objectives:  1) To ribotype isolates of Enterococcus faecalis from water and   suspected sources of contamination in order to develop a pathogen   tracker database and to validate that the indicator species has a   limited host range.  2) To use the database to identify the source(s) of fecal   contamination in the Wappinger and Stoney Creek tributaries  3) To develop a species-specific ribosomal DNA gene biosensor for   rapid detection of Enterococcus faecalis from environmental samples   (water and human feces).  4) To correlate detection limits of target DNA with the number of   Enterococcus faecalis cells/mL for usew in TDML studies in other   parts of New York State.    (microbial source tracking); (MST); (bacterial source tracking);   (BST); non-point source pollution; fecal contamination; water   quality; pathogens; wastewater

Status
In progress
Type
Project
Start Date
End Date
Researchers
Janice E. ThiesPrincipal Investigator
Associated with 1 projects
Agencies

Funding 15 projects for a total of $273,800.00
Scope of Study
Field Investigation
Laboratory Investigation
Resource Being Monitored
Emission / Release / Discharge / Waste Management
Human Health
Beneficial Use Impairment Assessments
Restrictions on Drinking Water Consumption or Taste and Odour Problems
Annex Numbers
Pollution from Contaminated Groundwater
Pollution from Non-Point Sources
Remedial Action Plans and Lakewide Management Plans
Monitoring
Annex
  • Annex Numbers
    Annex Numbers
    Pollution from Contaminated Groundwater
    Pollution from Non-Point Sources
    Remedial Action Plans and Lakewide Management Plans
  • Annex 17

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Great Lakes - St. Lawrence Region.