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Discrimination and characterization of environmental strains of Escherichia coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS)

A rapid and reliable bacterial source tracking (BST) method is   essential to counter risks to human health posed by fecal   contamination of surface waters. Genetic fingerprinting methods,   such as repetitive sequence based-PCR (rep-PCR), have shown promise   as BST tools but are time-consuming and labor-intensive. In this   work, we investigate the ability of MALDI-TOF-MS to characterize and   discriminate between closely related environmental strains of   Escherichia coli and to classify them according to their respective   sources. We compared the performance of a rapid MALDI-TOF-MS-based   method to a commonly used rep-PCR-based method that employs the BOX-  A1R primer. Among the criteria evaluated were repeatability and the   ability of each method to group E. coli isolates according to their   respective sources. Our data suggest that the MALDI-TOF-MS-based   approach has a lower repeatability level compared to rep-PCR but   offers an improved ability to correctly assign E. coli isolates to   specific source groups. In addition, we have identified five   biomarkers that appear conserved among avian species. We conclude   that MALDI-TOF-MS may represent a promising, novel and rapid   approach to addressing the problem of fecal contamination of surface   waters and warrants further investigation.    (microbial source tracking); (MST); (bacterial source tracking);   (BST)

Status
In progress
Type
Project
Start Date
End Date
Researchers
Rebecca A. GiebelPrincipal Investigator
Associated with 1 projects
Greg KleinheinzResearcher
Associated with 2 projects
Colleen McDermottResearcher
Associated with 2 projects
Wing H. HuenResearcher
Associated with 1 projects
Agencies
The National Science Foundation $ 700,000.00USDEstimates

Funding 1 projects for a total of $700,000.00

Funding 1 projects for a total of $5,000.00
Scope of Study
Laboratory Investigation
Scale of Phenomena
Cellular
Resource Being Monitored
Human Health

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