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A genomic and proteomic approach to characterizing natural variation in E. coli: Toward construction of a microbial source tracking database to identify sources of fecal water contamination in the State of Montana

The goal of this research is to conduct comprehensive genotypic and   phenotypic analyses on a series E. coli isolates from wild mammals   at all three levels of cellular information flow: genome,   transcriptome and proteome, and to apply the information to the   problem of tracking nonpoint sources of fecal water contamination.   Standard fingerprinting techniques (rep-PCR) as well as techniques   for looking for differences in transcriptome and proteome   composition (microarray based transcriptional profiling and 2D gel   electrophoresis) will be performed on E. coli isolates from several   different types of mammal host from Western Montana. Data will be   collected into a single relational database and analyzed using state-  of-the-art software to draw conclusions about the potential for   classification of unknown E. coli isolates from contaminated water   using a proteomics approach. The creation of a MST database for the   state of Montana will result in an enhanced ability of water quality   officials to identify and eliminate sources of fecal contamination   and greatly improve the health of our state waters.    (E. coli); (genomes); (fecal contamination); (waterborne diseases);   (microbial source tracking); (MST); (bacterial source tracking);   (BST) 

In progress
Start Date
End Date
William HolbenPrincipal Investigator
Associated with 1 projects
Margie KinnersleyResearcher
Associated with 1 projects

Funding 15 projects for a total of $273,800.00
Scope of Study
Field Investigation
Laboratory Investigation
Beneficial Use Impairment Assessments
Restrictions on Drinking Water Consumption or Taste and Odour Problems

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