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Development of a DNA Marker Gene System to Determine Sources of Fecal E. coli in Watersheds

While phenotypic and genotypic techniques have been used to   determine potential sources of fecal bacteria in surface waters,   most methods require the construction of large known-source   libraries, and often fail to adequately differentiate among   environmental isolates originating from diverse animal sources. In   contrast, library-independent, gene probe methods may provide a cost-  effective approach to determine sources of environmental isolates   originating from different animal sources. In this proposal, we will   use the suppression subtractive hybridization technique to enrich   for host source-specific DNA markers for E. coli originating from   cows and humans. We have previously used this approach to identify   DNA markers for E. coli originating from geese and have used these   markers as gene probes in a library independent analysis of sources   of E. coli in Lake Superior. The second objective of the proposed   research is to use the newly identified DNA markers (and the   existing goose marker gene) as gene probes to determine sources of   E. coli in the Minneopa Creek watershed. Analyses will be done using   a recently developed high throughput, automated, macroarray   screening procedure. The identified markers will provide a   quantitative, cost-effective, and accurate library-independent   method to determine sources of genetically diverse E. coli for use   in source tracking studies.    (microbial source tracking); (MST); (bacterial source tracking);   (BST); (E. coli); (gene probes); (robotic system); (fecal pollutants)

Status
In progress
Type
Project
Start Date
End Date
Researchers
Michael SadowskyPrincipal Investigator
Associated with 6 projects
Matthew J. HamiltonResearcher
Associated with 1 projects
Agencies

Funding 1 projects for a total of $42,908.00

Funding 15 projects for a total of $273,800.00
Scope of Study
Field Investigation
Laboratory Investigation
Lake Basin Connecting Channels
Lake Superior
State Province
Minnesota
Resource Being Monitored
Human Health
Annex Numbers
Pollution from Contaminated Groundwater
Pollution from Non-Point Sources
Remedial Action Plans and Lakewide Management Plans
General
Annex
  • Annex Numbers
    Annex Numbers
    Pollution from Contaminated Groundwater
    Pollution from Non-Point Sources
    Remedial Action Plans and Lakewide Management Plans
  • Annex 17

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