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Bear Creek Bacterial Source Tracking

This study investigated whether direct sequencing of a bacterial   gene could be utilized for detecting genetic differences between   bacterial strains for microbial source tracking. A 525-nucleotide   segment of the gene for B-glucuronidase (uidA) was sequenced in 941   Escherichia coli isolates from the Clinton River-Lake St. Clair   watershed, 182 E. coli isolates from human and animal feces, and 34   E. coli isolates from a combined sewer. Environmental isolates   exhibited 114 alleles in 11 groups on a genetic tree. Frequency of   strains from different genetic groups differed significantly (p <    . 3) between upstream reaches (Bear Creek-Red Run), downstream   reaches, and Lake St. Clair beaches. Fecal E. coli uidA sequences   exhibited 81 alleles that overlapped with the environmental set. An   algorithm to assign alleles to different host sources averaged   approximately 75% correct classification with the fecal data set.   Using the same algorithm, the percent of environmental isolates   assignable to humans decreased significantly between Bear Creek-Red   Run (3  ± 3%) and the beaches (17 ± 2%) (p <  . 5). Birds accounted   for approximately 5 % of assignable environmental isolates. For   combined sewer isolates, the same algorithm assigned 51% to humans.   These experiments demonstrate differences in the frequency of   different E. coli strains at different locations in a watershed, and   provide a "proof in principle" that sequence-based data can be used   for microbial source tracking.     (microbial source tracking); (MST); (bacterial source tracking);   (BST)

Status
In progress
Type
Project
Start Date
End Date
Researchers
Jeffrey RamPrincipal Investigator
Associated with 3 projects
Agencies

Funding 1 projects for a total of $0.00
Scope of Study
Field Investigation
Laboratory Investigation
Lake Basin Connecting Channels
Lake St. Clair
State Province
Michigan
Resource Being Monitored
Emission / Release / Discharge / Waste Management
Human Health
Beneficial Use Impairment Assessments
Beach Closings
Annex Numbers
Pollution from Contaminated Groundwater
Pollution from Non-Point Sources
Remedial Action Plans and Lakewide Management Plans
Surveillance and Monitoring
General
Monitoring
Annex
  • Annex Numbers
    Annex Numbers
    Pollution from Contaminated Groundwater
    Pollution from Non-Point Sources
    Remedial Action Plans and Lakewide Management Plans
    Surveillance and Monitoring
  • Annex 17

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