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Evaluation of microbial source tracking methods using mixed fecal sources in aqueous test samples

In this study, 22 researchers employing 12 different methods were   provided sets of identically prepared blind water samples. Each   sample contained one to three of five possible fecal sources (human,   dog, cattle, seagull or sewage). Researchers were also provided with   portions of the fecal material used to inoculate the blind water   samples for use as library material. No MST method that was tested   predicted the source material in the blind samples perfectly. Host-  specific PCR performed best at differentiating between human and non-  human sources, but primers are not yet available for differentiating   between all of the non-human sources. Virus and F+ coliphage  methods reliably identified sewage, but were unable to identify   fecal contamination from individual humans. Library-based isolate   methods correctly identified the dominant source in most samples,  but also had frequent false positives in which fecal sources not in   the samples were incorrectly identified as being present. Among the   library-based methods, genotypic methods generally performed better   than phenotypic methods.    This project was conducted by a governmental agency, so they did not   have a single grant that paid for the study.  There were about 12   sources that helped with the funding.  The total cost of the project   was around $45 K.    (microbial source tracking); (MST); (bacterial source tracking);   (BST)

Status
In progress
Type
Project
Start Date
End Date
Researchers
Stephen B. WeisbergPrincipal Investigator
Associated with 1 projects
John F. GriffithResearcher
Associated with 1 projects
Agencies

Funding 1 projects for a total of $0.00
Scope of Study
Laboratory Investigation
Resource Being Monitored
Emission / Release / Discharge / Waste Management
Human Health
Beneficial Use Impairment Assessments
Beach Closings
Restrictions on Drinking Water Consumption or Taste and Odour Problems

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