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National Risk Assessment of Microbiological Contaminants in Agricultural Run-off - Pilot Study (5 Yr Project)

The objective of this 5 year project, funded through the   Agricultural Policy Framework (APF), is to develop a better   understanding of the impact of various sources, including   agriculture, on microbiological water quality at selected drinking   water supply intake points. This study is being conducted in three   watersheds within Canada; South Nation River (Ontario), Oldman River   (Alberta), and Black Brook (New Brunswick). These watersheds  represent waters with various types of potential faecal impacts. In   order to characterize the nature and extent of faecal contamination   in these distinct watersheds, microbial source tracking (MST) methods  are being applied; relationships between faecal indicator bacteria   and waterborne pathogens of human health significance are also being   assessed. The MST methods being used include two library-based  methods [antibiotic resistance analysis (ARA) and rep-PCR] in the   South Nation watershed, and a library independent method   (Bacteroides PCR analysis) in all three watersheds. Faecal indicator  bacteria analyses include E. coli, thermotolerant coliforms,   Enterococcus, and in some instances Clostridium perfringens.   Bacterial pathogens of human health significance included in the   analyses are Salmonella, Shigella, Campylobacter, and E. coli   O157:H7. Project details, including watershed selection and   experimental approach, will be discussed. Pathogen prevalence and   preliminary results of MST will also be described.    (microbial source tracking); (MST); (bacterial source tracking);   (BST)

In progress
Start Date
End Date
Diane MedeirosPrincipal Investigator
Associated with 1 projects
Tom EdgeResearcher
Associated with 1 projects
Edward ToppResearcher
Associated with 2 projects

Funding 1 projects for a total of $4,800,000.00
Scope of Study
Field Investigation
Laboratory Investigation
Annex Numbers
Pollution from Contaminated Groundwater
Pollution from Non-Point Sources
Remedial Action Plans and Lakewide Management Plans
Research & Development
Annex 17
Mass transfer of pollutants between GL Basin Ecosystem components

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