Objectives: Bacterial Kidney Disease (BKD) is the most economically important disease of wild and farmed salmonids. Currently, there are no effective vaccines or treatments for BKD. Our overall objective is to develop BKD vaccines for fish. Our strategy is: 1) to use a combinatorial phage display library to identify novel antigens for BKD. 2) express those antigens in microalgae 3) deliver microalgae expressing BKD antigens to fish via feed or by immersion, and 4) to determine whether the microalgal-BKD antigen delivery system provides protection against BKD (vaccination). We propose that our vaccine development strategy will have broad applications for many disease systems including those in which it is not possible to isolate or grow the pathogen. Rationale: BKD is a major disease of fresh-water and marine salmonids resulting in substantial mortality. The disease is slow to manifest symptoms and therefore is difficult to treat with antibiotics. As a result, BKD is a prime candidate for the development of an effective vaccine treatment. To date, traditional approaches to identifying and delivering vaccines to trout for BKD control have proven ineffective. We propose that a combinatorial antigen discovery strategy will lead to the rapid identification of novel vaccine immunogens. The delivery of these immunogens in microalgae will induce an immune response both in serum and in mucus, providing the best possible protection against BKD.
- Reversible Inhibition of Spreading of In Vitro Infection and Imbalance of Viral Protein Accumulation at Low pH in Viral Hemorrhagic Septicemia Rhabdovirus, a Salmonid Rhabdovirus
- Weevil Attractants Released by Eurasian Watermilfoil
- Identification of Small Molecular Weight Factors that Bind HeavyMetals in Algae
- Genetic Evaluation of Coaster Brook Trout Hatchery Programs Using DNA Markers
- Critical Elements of Brook Trout Habitat in Lake Superior
- Selective breeding provides an approach to increase resistanceof rainbow trout (Onchorhynchus mykiss) to the diseases, enteric redmouth disease, rainbow trout fry syndrome, and viral haemorrhagic septicaemia
- In-situ Biosensors for Monitoring of Fish Physiology and Behavior via Acoustical Telemetry
- Dispersal and Fate of Sediments from the Nemadji River, Lake Superior
- Development of Fluorescent Biosensors for In Situ Trace-Metal Quantification and Biomonitoring
- Expression of inducible CC chemokines in rainbow trout (Oncorhynchus mykiss) in response to a viral haemorrhagic septicemia virus (VHSV) DNA vaccine and interleukin 8
General
Annex
General
- Scope of Study
Scope of StudyLaboratory Investigation
- Scale of Phenomena
Scale of PhenomenaCellular
- Impact of Pollutants
- Processes
- Land Use and Habitat
- Resource Management
Resource ManagementFisheries
- Socio-Economic
- State / Province
State ProvinceOhio
- Lake Basin / Connecting Channels
Monitoring
Aquatic Nuisance Species
Annex
- Annex Numbers
Annex NumbersResearch & Development
- Annex 17