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Reversible Inhibition of Spreading of In Vitro Infection and Imbalance of Viral Protein Accumulation at Low pH in Viral Hemorrhagic Septicemia Rhabdovirus, a Salmonid Rhabdovirus

The inhibition of viral hemorrhagic septicemia rhabdovirus (VHSV) in   vitro infection by pHs of <7 (low pH) has been previously reported.   Nevertheless, the details of the mechanism underlying this effect   remain obscure. We present evidence showing that low-pH inhibition   occurs during a viral postadsorption step. Thus, while VHSV bound,   replicated within single cells, and presented its G protein on the   membranes of infected cells at both low and physiological pHs, both   cell-to-cell spreading of infection (as estimated by the appearance   of foci of infected cells) and fusion (as estimated by a syncytium   assay) were inhibited by this low pH. The decreased VHSV titers and   the inhibition of both cell-to-cell spreading of infection and   fusion could be reversed by adjusting the pH to 7.5 at any time   during infection. This effect should be taken into account to avoid   false negatives in the diagnosis of VHSV by cell culture. On the   other hand, the cell-to-cell spreading of infection at pH 7.5 could   be stopped at any time by reducing the pH to 6.5. Since at low pH   there were changes in the protein G conformation and smaller and   imbalanced amounts of N with respect to M1, M2, and G viral  proteins, alterations of the assembly and/or budding of VHSV are   most probably involved in the absence of newly released infective   virions.    (VHS); (VHSv); (viral hemorrhagic septicemia); (viral haemorrhagic   septicaemia); (viral hemmorrhagic septicemia)

Status
In progress
Type
Project
Start Date
End Date
Researchers
A. FalcoPrincipal Investigator
Associated with 1 projects
Julio CollResearcher
Associated with 1 projects
Luis PerezResearcher
Associated with 1 projects
Agencies
INIA-SIGT, Biotecnologia $ 0.00USDEstimates

Funding 1 projects for a total of $0.00
Scope of Study
Laboratory Investigation
Scale of Phenomena
Cellular
Organism
Impact of Pollutants
Exotic Species
Resource Management
Fisheries
Resource Being Monitored
Fisheries
Beneficial Use Impairment Assessments
Fish Tumours or Other Deformities
Loss of Fish and Wildlife Habitat
Tainting of Fish and Wildlife Populations
Biology And Life History
Parasites and Diseases
Annex Numbers
Research & Development
Annex 17
Impact of water quality and AIS on fish and wildlife populations and habitats
General
Monitoring
Annex
  • Annex Numbers
    Annex Numbers
    Research & Development
  • Annex 17
    Annex 17
    Impact of water quality and AIS on fish and wildlife populations and habitats

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