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Rapid concentration and quantification of bacteria and viruses from marine waters

Project objectives:   1) Confirm the ability of a mobile hollow fiber ultrafiltration   system (Rec DEC) coupled with immunosensor detection by the 4S   RAPTOR for rapid, quantitative detection of enterococci in   California marine waters (previously demonstrated in Florida).   2) Confirm the ability to recover culturable Enterococcus spp. cells   from the ultrafiltration/RAPTOR system.   3) Quantify Enterococcus spp. cells using the ultrafiltration/RAPTOR   system in samples collected concurrently with the SCCWRP   epidemiology study.   4) Elute cells from RAPTOR waveguides followed by enumeration of   total Enterococcus and enrichment/PCR quantification of Ent. faecium   carrying the esp gene.   5) Quantify human polyomaviruses (viral indicators of human sewage)   in samples collected concurrently with the SCCWRP epidemiology study   by a) elution from hollow fiber filters and b) conventional   filtration of 6   ml samples.  6) Analyze data in the context of the epidemiology results to   determine correlation of results with public health outcomes.     (microbial source tracking); (MST); (bacterial source tracking);   (BST)

Status
In progress
Type
Project
Start Date
End Date
Researchers
Valerie J. HarwoodPrincipal Investigator
Associated with 4 projects
Daniel V. LimResearcher
Associated with 1 projects
Agencies
NOAA-CICEET $ 145,893.00USDActual

Funding 1 projects for a total of $145,893.00

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Great Lakes - St. Lawrence Region.