Skip to main content
hyi
Banner
banner
bn

BIOASSAY DIRECTED ISOLATION AND CHARACTERIZATION OF A NATURAL SEA LAMPREY DETERRENT

A chemical deterrent derived from sea lamprey tissue (hypothesized as an alarm cue) has proven highly effective when used to guide migrating sea lampreys to one side of a stream (Bals et al., in review), and to the entrance of a barrier-integrated trap (Hume et al., in review). The magnitudes of these effects on migrant behavior were substantially greater than those observed with chemical attractants (pheromones). In order to develop full-scale control applications based on chemical deterrence, the chemical identity of the lamprey alarm cue compound(s) must be discovered and behavioral reactivity confirmed (per FIFRA requirements). Preliminary Data. Using bioassay-guided differential solvent extractions including ethanolic (full extract and six fractions), methanolic (soluble and insoluble), and ethyl acetate (three fractions), we confirmed behavioral reactivity distributed across several ethanolic fractions. Specifically, sea lampreys most strongly avoided the odors emitted from chloroform soluble ethanolic extract derived from lyophilized skin. Silica gel thin layer chromatography (TLC) and high pressure liquid chromatography (HPLC) profiles of the bioactive fractions indicated a mixture of potential alarm cue compounds. We propose to conduct a bioassay-guided fractionation and purification of the aqueous ethanolic extract of adult sea lamprey skin and determine the chemical identity of the active compound(s) using spectral and chemical methods. Therefore, our objectives of thisproject will be (1) Isolate and purify deterrent compounds from lyophilized sea lamprey skin via bioassay-guided fractionation. (2) Determine the chemical identity of the active compound(s) using spectral and chemical methods. (3) Produce sufficient quantities of purified active compound(s) for confirmation testing in raceways and streams. 
 

Status
In progress
Type
Project
Start Date
End Date

The Great Lakes - St. Lawrence Research Inventory is an
interactive, Internet-based, searchable database created as a tool to collect and disseminate
up-to-date information about research projects in the
Great Lakes - St. Lawrence Region.