Inbred lines differentially susceptible to diseases are a powerful tool to get insights into the mechanisms of genetic resistance to pathogens. In fish, chromosome manipulation techniques allow a quick production of such homozygous lines. Using gynogenesis, we produced nine homozygous clones of rainbow trout from a domestic population (INRA Sy strain). We examined the variability between clones for resistance to two rhabdoviruses, the viral haemorrhagic septicaemia virus (VHSV) and the infectious haematopoietic necrosis virus (IHNV). Intraperitoneal injections and waterborne infections were performed in parallel for both viruses. No survival was recorded after intraperitoneal injection of VHSV or IHNV, indicating that fish from all clones were fully susceptible to both viruses by this route of infection. In contrast, the different clones showed a wide range of survival frequency after waterborne infection. The resistance levels to VHSV ranged from to 99% and resistance was not abrogated when resistant and sensitive animals were mixed and subjected to waterborne infection. VHSV was recovered from 1 % of resistant fish after waterborne infection, confirming that virus replication was possible in this context but effective only in a low proportion of the population. The different clones also exhibited a wide range of survival ( to 68%) after a waterborne infection with IHNV. Although VHSV-resistant clones were not fully resistant to IHNV, the susceptibility to IHNV and VHSV tended to be correlated, suggesting that non-specific mechanisms common to both viruses were involved. (VHS); (VHSv); (viral hemorrhagic septicemia); (viral haemorrhagic septicaemia); (viral hemmorrhagic septicemia); (infectious haematopoietic necrosis virus); (IHNV)
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General
Monitoring
Aquatic Nuisance Species
Annex
General
- Scope of Study
Scope of StudyLaboratory Investigation
- Scale of Phenomena
Scale of PhenomenaCellularOrganism
- Impact of Pollutants
Impact of PollutantsExotic Species
- Processes
- Land Use and Habitat
- Resource Management
Resource ManagementFisheries
- Socio-Economic
- State / Province
- Lake Basin / Connecting Channels
Monitoring
- Purpose of Project
- Frequency
- Number of Sampling Entities
- Data Availability
- Region Being Monitored
- Resource Being Monitored
Resource Being MonitoredFisheries
- Program Drivers
- Beneficial Use Impairment Assessments
Beneficial Use Impairment AssessmentsFish Tumours or Other DeformitiesLoss of Fish and Wildlife HabitatTainting of Fish and Wildlife Populations
Aquatic Nuisance Species
- Biology And Life History
Biology And Life HistoryParasites and Diseases
- Control And Mitigation
- Ecosystem Effects
- Prevention and Introduction
- Socio-economic Consideration and Analysis
- Spread of Established ANS Populations
Annex
- Annex Numbers
Annex NumbersResearch & Development
- Annex 17
Annex 17Impact of water quality and AIS on fish and wildlife populations and habitats