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Time course study of in situ expression of antigens following DNA-vaccination against VHS in rainbow trout (Oncorhynchus mykiss Walbaum) fry

This study was performed as a time course study of fish vaccinated   with 2  mg plasmid DNA vaccine encoding either the VHSV G-protein or   the VHSV N-protein. Samples of the injection site were collected   sequentially over a 7-week period. The study revealed an intense   positive staining by immunohistochemistry for the viral G-protein  mainly in the membrane of intact myocytes, most prominent by days 1    to 27, and with concomitant infiltration of inflammatory cells by   days 13 to 38 that subsequently lead to a marked reduction in the   number of myocytes expressing the G-protein. By immunofluorescence,   infiltrating cells positive for MHC II, IgM, and C3 were   demonstrated. By contrast, in fish vaccinated with the VHSV-N   construct, fewer, diffusely positive myocytes were found, most   prominent by days 13 to 38, these having a positive reaction for the   N-protein mainly in the cytoplasm and variably in the membrane. N-  protein positive myocytes did not attract infiltrating cells to the   same degree. Positive reaction for the N-protein almost ceased by   day 48 post-vaccination.    (VHS); (VHSv); (viral hemorrhagic septicemia); (viral haemorrhagic   septicaemia); (viral hemmorrhagic septicemia)

Status
In progress
Type
Project
Start Date
End Date
Researchers
Ellen LorenzenPrincipal Investigator
Associated with 1 projects
Katja Einer-JensenResearcher
Associated with 2 projects
Bjorn BrudesethResearcher
Associated with 3 projects
Oystein EvensenResearcher
Associated with 4 projects
Agencies

Funding 1 projects for a total of $40,000.00
Scope of Study
Laboratory Investigation
Scale of Phenomena
Cellular
Organism
Impact of Pollutants
Exotic Species
Resource Management
Fisheries
Resource Being Monitored
Fisheries
Beneficial Use Impairment Assessments
Fish Tumours or Other Deformities
Loss of Fish and Wildlife Habitat
Tainting of Fish and Wildlife Populations
Biology And Life History
Parasites and Diseases
Annex Numbers
Research & Development
Annex 17
Impact of water quality and AIS on fish and wildlife populations and habitats
General
Monitoring
Annex
  • Annex Numbers
    Annex Numbers
    Research & Development
  • Annex 17
    Annex 17
    Impact of water quality and AIS on fish and wildlife populations and habitats

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