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Gene silencing technologies to control sea lamprey: a proof-of-concept

The genome of the sea lamprey is currently being sequenced, and it   is anticipated that the wealth of information derived from this   sequence database will ultimately permit researchers to identify   novel molecular targets to control this pest species. While the   function of some newly sequenced genes can be deduced by comparisons   with genes of other species, the function of many of the lamprey's   genes will need to be experimentally determined. RNA interference   (RNAi) is a method of selectively silencing the expression of any   gene by introducing double-stranded RNA (dsRNA) into a cell. By   developing novel, higher throughput dsRNA delivery methods for   lampreys, we can use RNAi to produce loss-of-function mutations to   discover the function of new lamprey genes. In addition to using   RNAi as a molecular biology tool, we also intend to test whether   dsRNA can be delivered to lamprey larvae in food formulations.   Ingestion of dsRNA has proven to be effective in silencing genes   within gut cells of a range of other species. In this project, we   will assess whether orally-delivered dsRNAs can specifically silence   genes critical for gut function, in an effort to develop dsRNA as a   novel lampricide. By selecting gene sequences that are unique to sea   lampreys, it may be possible to design dsRNAs that selectively kill   sea lamprey larvae without adversely affecting benign or non-target   species.

Status
In progress
Type
Project
Start Date
End Date
Researchers
Steve WhyardPrincipal Investigator
Associated with 1 projects
Margaret DockerResearcher
Associated with 2 projects
Agencies
Great Lakes Fishery Commission $ 93,000.00CADEstimates

Funding 54 projects for a total of $5,089,137.00
Scope of Study
Laboratory Investigation
Scale of Phenomena
Biochemical
Cellular
Organism
Lake Basin Connecting Channels
Lake Superior
St. Marys River
State Province
Michigan
Minnesota
Ontario
Wisconsin
Biology And Life History
Genetics
Control And Mitigation
Chemical Measures
Annex Numbers
Research & Development
Annex 17
Impact of water quality and AIS on fish and wildlife populations and habitats
General
Annex
  • Annex Numbers
    Annex Numbers
    Research & Development
  • Annex 17
    Annex 17
    Impact of water quality and AIS on fish and wildlife populations and habitats

The Great Lakes - St. Lawrence Research Inventory is an
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up-to-date information about research projects in the
Great Lakes - St. Lawrence Region.