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Rapid Detection of Toxic and Taste and Odor Causing Cyanobacteria in Indiana Surface Water

The goal of this project is to develop a molecular detection method   that is specific to these two species: (Cylindrospermopsis   raciborskii and Pseudanabaena limnetica), is sensitive at low cell   concentrations, is rapid, and is relatively economical. Although PCR   has been used to amplify cyanobacterial DNA, and specific primers   have been created to detect certain species in water, these   techniques are qualitative, not quantitative. The researchers   propose developing a quantitative detection method using 5'-   exonuclease PCR, which measures the release of a fluorescent dye in   response to amplification of a specific target sequence. A properly   developed and calibrated assay will allow the quantitative   determination of the cyanobacterial cell concentration. This   technique has been widely used in medical research but has seen only   limited use in environmental analysis. The successful development of   this technology to rapidly detect and monitor small numbers of cells   before they reach peak populations has the potential to improve the   management of cyanobacteria and other HABs not only locally but   nationally, and even globally.    (algal bloom); (algal blooms); (harmful algal bloom); (harmful algal   blooms); (HAB's); (HABs); (HAB); (eutrophication); (cyanobacteria);   (blue-green algae); (human health); (drinking water); (restrictions   on drinking water); (AIS); (aquatic invasive species); (ANS);   (aquatic nuisance species); (polymerase chain reaction); (PCR)

Status
In progress
Type
Project
Start Date
End Date
Researchers
Carole A. LembiPrincipal Investigator
Associated with 1 projects
Agencies
U.S. Geological Survey $ 0.00USDEstimates

Funding 13 projects for a total of $538,321.00
Scope of Study
Field Investigation
Laboratory Investigation
Scale of Phenomena
Biochemical
Physical/Chemical
State Province
Indiana
Spread of Established ANS Populations
Predictive Models
Socio-economic Consideration and Analysis
Human Health Aspects
Annex Numbers
Remedial Action Plans and Lakewide Management Plans
Research & Development
Annex 17
Cause-effect inter-relationships of productivity and ecotoxicity
Impact of water quality and AIS on fish and wildlife populations and habitats
General
Annex
  • Annex Numbers
    Annex Numbers
    Remedial Action Plans and Lakewide Management Plans
    Research & Development
  • Annex 17
    Annex 17
    Cause-effect inter-relationships of productivity and ecotoxicity
    Impact of water quality and AIS on fish and wildlife populations and habitats

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Great Lakes - St. Lawrence Region.