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Effects of lampricides on target and non-target species: from protein expression to ecological consequences

RATIONALE:  Although lampricides such as 3-trifluoromethyl-4-  nitrophenol (TFM) and granular niclosamide have been used   successfully for the control of sea lamprey in the Great Lakes   region, their modes of toxic action are not completely understood.    In addition, their effects on non-target fish species are not   clear.  Historically, toxicant effects on aquatic organisms have   primarily been evaluated via lethality.  While modern approaches of   toxicant and stress evaluation routinely involve non-lethal   endpoints, many such indices are either too coarse to truly   understand mechanisms of action or are not sufficiently ecologically   relevant to facilitate elucidation of population level effects.   Herein, we propose the simultaneous use of molecular (proteomics and   metabolomics) and physiological (growth and behavior) approaches as   a way to increase our understanding on both modes of toxic action of   TFM as well as their potential sub-lethal effects on non-target   species.      OBJECTIVES: 1) Elucidate the modes of toxic action of TFM and   niclosamide in sea lamprey through characterization of protein and   metabolite expression; 2) Compare lampricide-induced protein and   metabolite expression in sea lampreys to those observed in non-  target fish species (lake sturgeon and rainbow trout); and 3)   Evaluate if lampricide exposure of young non-target fish has   ecologically meaningful sub-lethal effects.    METHODS:  All experiments will be conducted at Purdue?s Baker   Aquaculture Center. 1) We will conduct a series of exposure   experiments with age-  sea lamprey, lake sturgeon, and rainbow   trout.  These experiments will include different concentrations of   lampricides. 2) At the end of the exposure period, fish livers will   be flash frozen for subsequent proteomic and metabolomic analyses in   order to map the expression of up to several hundred proteins and   metabolites. 3) Finally, we will undertake a second set of   experiments through which we will compare ecologically relevant   effects of lampricides (survival, growth, condition, and predatory   avoidance) on lake sturgeon, rainbow trout, and smallmouth bass.    

Status
In progress
Type
Project
Start Date
End Date
Researchers
Maria S. SepulvedaPrincipal Investigator
Associated with 5 projects
Agencies
Great Lakes Fishery Commission $ 127,540.00USDEstimates

Funding 54 projects for a total of $5,089,137.00
Scope of Study
Laboratory Investigation
Scale of Phenomena
Biochemical
Cellular
Organism
Physical/Chemical
Population
Impact of Pollutants
Exotic Species
Toxic Organics
Resource Management
Fisheries
Lake Basin Connecting Channels
Lake Erie
Lake Huron
Lake Michigan
Lake Ontario
Lake Superior
State Province
Indiana
Biology And Life History
Physiology and Behavior
Control And Mitigation
Chemical Measures
Annex Numbers
Research & Development
Annex 17
Impact of water quality and AIS on fish and wildlife populations and habitats
General
Annex
  • Annex Numbers
    Annex Numbers
    Research & Development
  • Annex 17
    Annex 17
    Impact of water quality and AIS on fish and wildlife populations and habitats

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