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ESTABLISHMENT OF A PCR-BASED METHOD FOR FERTILITY ASSESSMENT IN STERILE MALE SEA LAMPREY

The sterile-male-release technique (SMRT) is a key alternative   control strategy used to manage sea lampreys in the Great Lakes.  To   achieve sterilization, male sea lampreys are injected with the   chemosterilant bisazir, which destroys genes essential for embryo   development of offspring.  While bisazir has proved to be an   effective sterilant for male sea lampreys, there is the potential   for problems to occur during the sterilization process that would   prevent males from being sterilized.  During the past, laboratory   fertilizations were used to verify fertility, but because the   effects of bisazir can only be observed during later stages of   embryogenesis, this technique was found impractical and has not been   conducted in over a decade.  More recently, the single cell gel   electrophoresis assay or Comet assay has been explored as a way to   assay fertility, but this method also has limitations in that the   comet assay may fail to detect damaged DNA, and still requires that   males be held until spermiation (which can take weeks) before the   assay can be performed.  To prevent the release of fertile males   (and the subsequent failure of the SMRT), a quality assurance method   that can quickly and accurately assess fertility in male sea   lampreys needs to be developed.   This project will develop a PCR based method to test the integrity   of house-keeping sequences, bisazir targets sequences, or key genes   for embryogenesis, which will assess the fertility of bisazir   treated lampreys as a rapid and less expensive quality control   protocol for SMRT 

Status
In progress
Type
Project
Start Date
End Date
Researchers
Ziping ZhangPrincipal Investigator
Associated with 1 projects
Michael SiefkesResearcher
Associated with 2 projects
Ronald WalterResearcher
Associated with 1 projects
Agencies
Great Lakes Fishery Commission $ 150,000.00USDEstimates

Funding 54 projects for a total of $5,089,137.00
Scope of Study
Laboratory Investigation
Scale of Phenomena
Biochemical
Impact of Pollutants
Exotic Species
Processes
Treatment / Manufacturing Processes
Resource Management
Fisheries
Lake Basin Connecting Channels
Lake Huron
State Province
Michigan
Annex Numbers
Research & Development
Annex 17
Impact of water quality and AIS on fish and wildlife populations and habitats
General
Annex
  • Annex Numbers
    Annex Numbers
    Research & Development
  • Annex 17
    Annex 17
    Impact of water quality and AIS on fish and wildlife populations and habitats

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Great Lakes - St. Lawrence Region.