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Establishing Physiological Indices for More Effective Use of TFM toControl Sea Lamprey Populatins in the Great Lakes.

The goals of this project are to: (I) Identify factors that explain  the greater sensitivity of sea lamprey (Petromyzon marinus) to the  lampricide 3-trifluoromethyl-4-nitrophenol (TFM) compared to  non-target fishes, (II) Determine if TFM has reversible physiological  effects on lampreys and non-target fishes, (III) Ascertain how  internal fuel stores and season affect TFM sensitivity in lampreys and  non-target fishes. In addition to lamprey, non-target fishes to be  used will include TFM-tolerant rainbow trout (Oncorhynchus mykiss) and  TFM-sensitive lake sturgeon (Acipenser fulvescens). The hypothesis  that the greater TFM sensitivity of lampreys and lake sturgeon  compared to trout is primarily due to their inability to detoxify TFM  will be tested by exposing animals to TFM and relating survival and  internal fuel stores to amounts of TFM and TFM metabolites in the  fish?s body during and after exposure. As interspecies differences in  TFM uptake may explain variation in TFM sensitivity, TFM uptake rates  will also be determined. The hypothesis that TFM directly targets  gills will be investigated by measuring Na+/K+ ATPase activity, ion  uptake by the gills, and by examining gill morphology. The prediction  that TFM has reversible sub-lethal effects in non-target fishes but  not lampreys will be examined by measuring glycogen and other fuels in  the body during and following exposure to TFM. Swim performance will  also be evaluated in trout and sturgeon to determine if this  ecologically important behaviour is impaired by TFM.  Through fasting  studies we will also determine if it is possible to use fuel store  levels in the body to predict TFM sensitivity in lamprey and  non-target fishes. To further examine this possibility, larval lamprey  will be collected during spring, summer and fall, and their TFM  sensitivity related to their internal fuel stores and condition  factor.  In summary, this research will identify factors that  influence TFM treatment effectiveness, better explain the non-target  effects of TFM, and further our understanding of the mode of TFM  toxicity. Physiological criteria that can be used to predict when and  why sea lamprey and non-target fishes are most sensitive to TFM may  also make it possible to modify lampricide application schedules to  maximize TFM effectiveness, while reducing non-target effects. 

Status
In progress
Type
Project
Start Date
End Date
Researchers
Michael WilkiePrincipal Investigator
Associated with 2 projects
Roger BergstedtResearcher
Associated with 7 projects
Gordon McDonaldResearcher
Associated with 3 projects
Agencies
Great Lakes Fishery Commission $ 142,361.00CADEstimates

Funding 54 projects for a total of $5,089,137.00
Scope of Study
Field Investigation
Laboratory Investigation
Scale of Phenomena
Biochemical
Cellular
Organism
Impact of Pollutants
Exotic Species
Processes
Treatment / Manufacturing Processes
Land Use and Habitat
Shoreline
Uplands
Resource Management
Fisheries
State Province
Michigan
Ontario
Biology And Life History
Physiology and Behavior
Control And Mitigation
Integrated Control Strategy
Annex Numbers
Research & Development
General
Annex

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Great Lakes - St. Lawrence Region.