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Near-real Time, Highly Sensitive and Selective Field Deployable Biosensor for Cyanotoxins and Cyanobacteria Using both Antibodies and DNA-signatures

Objectives:   1) Develop a batch cyanotoxin measurement method for field use   preserving the previously achieved detection sensitivity of PEMC   sensors, and validate measurements with current methods (ELISA and   HPLC/MS);  2) Develop a new rapid method for anatoxin-a and anatoxin-a(s) in   source and finished water matrices based on an available, but weakly   binding, antibody and an enzyme-binding assay;  3) Develop a rapid DNA-based assay for cyanobacteria, and;  4) Test lake water samples analyzed by US Geological Survey (USGS)   using the method developed in the first three objectives.    (cyanobacteria); (cyanotoxin); (cyanotoxins); (toxin); (toxins);   (drinking water); (restrictions on drinking water); (anatoxins);   (anatoxin); (biosensors); (biosensor); (human health); (toxins in   drinking water); (toxin in drinking water)

Status
In progress
Type
Project
Start Date
End Date
Researchers
Raj MutharasanPrincipal Investigator
Associated with 1 projects
Agencies
Environmental Protection Agency $ 600,000.00USDEstimates

Funding 21 projects for a total of $7,345,444.00
Scope of Study
Laboratory Investigation
Scale of Phenomena
Biochemical
Physical/Chemical

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