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Evaluation of Enterococci and Bacteroides Real Time PCR Assays for Measuring Recreational Water Quality in Iowa with a Source Tracking Perspective

Enterococci are recognized by the U.S. EPA as indicator organisms to   assess water quality in fresh and marine waters. Although   enterococci are a robust indicator of fecal contamination in marine   waters, interpretation of elevated levels in fresh waters is less   clear. Because enterococci can originate from plants or sewage, it   is important to understand the source of the enterococci as it   relates to swimmers' health. It may be that the species of   Enterococcus present, rather than total Enterococcus spp., more   accurately indicates the public health risk. This project will   evaluate four real-time qPCR methods for enterococci and Bacteriodes   and apply these methods to monitor ten Iowa beaches with elevated   levels of enterococci and/or E. coli. This study will determine if   the rapid total enterococci qPCR method can serve as a surrogate for   the standard EPA culture method and if all four rapid tests will be   able to track the source of the enterococci.    (enterococci); (microbial source tracking); (MST); (BST); (bacterial   source tracking); (beach closures); (beach closure); (beach   closings); (beach closing); (qPCR); (PCR); (quantitative polymerase   chain reaction); (polymerase chain reaction); (Iowa)

Status
In progress
Type
Project
Start Date
End Date
Scope of Study
Field Investigation
Laboratory Investigation
Scale of Phenomena
Biochemical
Physical/Chemical
Land Use and Habitat
Shoreline
Resource Being Monitored
Human Health
Beneficial Use Impairment Assessments
Beach Closings
Annex Numbers
Pollution from Contaminated Groundwater
Remedial Action Plans and Lakewide Management Plans
General
Annex
  • Annex Numbers
    Annex Numbers
    Pollution from Contaminated Groundwater
    Remedial Action Plans and Lakewide Management Plans
  • Annex 17

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up-to-date information about research projects in the
Great Lakes - St. Lawrence Region.