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Evaluation of Adenovirus Real-Time and Conventional PCR Assays to Detect Fecal Contamination in Water and to Identify Its Source

Fecal contamination of water is a significant public health concern.   The primary objective of this proposal is to determine the   usefulness of adenovirus as an indicator of fecal contamination and   its potential for fecal sourcetracking. This objective will be   addressed by the following aims: (1) to improve upon and validate a   PCR algorithm to detect human and animal adenoviruses in fecal waste   and water, (2) to conduct surveys of adenoviruses in cattle and   swine stool in order to determine the usefulness of adenovirus as a   source-tracking organism, and (3) to estimate the prevalence of   human and animal adenoviruses at impaired and transitional Iowa   beaches and to identify parameters associated with adenovirus in   surface water. Results from this study will be used to further   develop rapid methods to detect adenoviruses in water and accurately   identify sources of contamination which would be informative to risk   assessment and risk management practices.    (adenovirus); (adenoviruses); (microbial source tracking); (MST);   (BST); (bacterial source tracking); (beach closures); (beach   closure); (beach closings); (beach closing)

Status
In progress
Type
Project
Start Date
End Date
Scope of Study
Field Investigation
Laboratory Investigation
Scale of Phenomena
Biochemical
Physical/Chemical
Land Use and Habitat
Shoreline
Resource Being Monitored
Human Health
Beneficial Use Impairment Assessments
Beach Closings
Annex Numbers
Pollution from Contaminated Groundwater
Remedial Action Plans and Lakewide Management Plans
Research & Development
Annex 17
Physical and transformational processes affecting delivery of pollutants
General
Annex
  • Annex Numbers
    Annex Numbers
    Pollution from Contaminated Groundwater
    Remedial Action Plans and Lakewide Management Plans
    Research & Development
  • Annex 17
    Annex 17
    Physical and transformational processes affecting delivery of pollutants

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Great Lakes - St. Lawrence Region.