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Phylogenetic analysis and physiological characterization of photosynthetic picoplankton in Lake Erie

The two year study will evaluate the phylogeny of the Ppico   assemblage, with respect to relevant in-lake gradients that we know   exist (light, temperature, oxygen). For example, we will analyze the   taxonomic composition of the Ppico community before and after the   onset of central basin anoxia using both direct counts and molecular   tools described below. Ppico populations will be studied by sampling   primarily central basin stations. Samples will be assayed using both   ecophysiological measurements (pigments, oxygen and nutrients), and   molecular phylogenetics (PCR of 16S rDNA, 16S-23S ITS and rbcL   sequences with quantification by qPCR). PCR amplicons of sequences   (16S, 16-23S ITS) from samples collected throughout the lake will be   compared to identify the relatedness of these sequences to known   sequences from marine and freshwater environments. Quantitative PCR   (qPCR) with cyanobacterial specific and Prochlorococcus 16-23S ITS   primers will reveal the relative abundance of the individual members   of the Ppico assemblage, which in turn will be compared with water   column estimates derived using direct counts. Culturing of the   abundant Ppicos from the hypoliminion prior and after anoxia will   allow physiological studies aimed at determining their   photosynthetic performance in response to temperature, light and   nutrients.    (eutrophication); (anoxic); (anoxia); (picoplankton); (Lake Erie);   (Ppico); (plankton); (algal bloom); (algal blooms)

In progress
Start Date
End Date
George BullerjahnPrincipal Investigator
Associated with 3 projects
Robert McKayResearcher
Associated with 1 projects
Ohio Sea Grant College Program $ 116,608.00USDEstimates

Funding 15 projects for a total of $599,045.00
State of Ohio $ 86,678.00USDEstimates

Funding 8 projects for a total of $223,131.00
Scope of Study
Field Investigation
Laboratory Investigation
Scale of Phenomena
Impact of Pollutants
Nutrients, Including Phosphorus
Lake Basin Connecting Channels
Lake Erie
State Province

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