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Investigating chronic toxicity and bioaccumulation of microcystins in freshwater fishes using toxicogenomics and histopathology

Investigating Chronic Toxicity and Bioaccumulation of Microcystins   in Freshwater Fishes Using Toxicogenomics and Histopathology. This   project is part of the interagency Ecology and Oceanography of   Harmful Algal Blooms (ECOHAB) program. During the last 1  years,   Microsystis spp. blooms have occurred in Western Lake Erie, and   elevated levels of microcystins have become a concern for both human   and ecosystem health. Our objective is to investigate the   predominant microcystin found in this system (microcystin-LR) in   model fish species and to relate laboratory results to chronic low-  level toxin exposure and bioaccumulation found in higher trophic   level fish in W. Lake Erie. We hypothesize that (1) specific genes   that respond to microcystin-LR exposure in larval and adult   zebrafish can be identifed and selected as biomarkers; (2) effects   of chronic, low concentration exposure of microcystin-LR can be   detected by changes in biomarker gene expression, tissue histology   and reproduction in zebrafish; (3) bioaccumulation of microcystin in   channel catfish is affected by route of expusure and effects can be   detected in biomarker gene expression and histopathology; and (4)   bioaccumulation and effects of chronic low, concentration exposure   to microcystins can be detected in higher trophic level fish   collected from W. Lake Erie by tissue analysis and the evaluation of   biomarkers resolved from alb and mesocosm experiments.    Genes selected from microarray experiments will improve our   understanding of the mechanisms of microcystin toxicity and enable   more specific probing into the factors that influence   bioaccumulation and toxicity in fish via in vitro, mesocosm, and in   situ approaches. Our focus on chronic, low-concentration exposures   to will begin to address an important knowledge gap regarding the   long-term effects of algal toxins on ecological health. We expect to   determine toxin concentrations that cause negative effects in fish   during chronic exposure and to demonstrate toxicogenetic and   histopathological approaches that can be employed in ecological   forecasting of system health.    (microcystin); (microcystins); (eutrophication); (harmful algal   blooms); (algal blooms); (algal bloom); (harmful algal bloom);   (HAB); (HAB's); (HABs); (Lake Erie); (bioaccumulation); (human   health); (restrictions on fish consumption); (fish consumption);   (zebrafish); (blue-green algae); (toxin); (toxins)

In progress
Start Date
End Date
Theodore B. HenryPrincipal Investigator
Associated with 1 projects
Steven WilhelmResearcher
Associated with 2 projects
Gary S. SaylerResearcher
Associated with 1 projects
Richard J. StrangeResearcher
Associated with 1 projects
NOAA $ 145,655.00USDEstimates

Funding 11 projects for a total of $502,508.00
Scope of Study
Field Investigation
Laboratory Investigation
Scale of Phenomena
Impact of Pollutants
Nutrients, Including Phosphorus
Resource Management
Lake Basin Connecting Channels
Lake Erie
State Province
New York
Resource Being Monitored
Human Health
Plankton / Microorganisms
Beneficial Use Impairment Assessments
Eutrophication or Undesirable Algae
Loss of Fish and Wildlife Habitat
Restrictions of Fish and Wildlife Consumption
Tainting of Fish and Wildlife Populations
Annex Numbers
Pollution from Non-Point Sources
Research & Development
Annex 17
Cause-effect inter-relationships of productivity and ecotoxicity
  • Annex Numbers
    Annex Numbers
    Pollution from Non-Point Sources
    Research & Development
  • Annex 17
    Annex 17
    Cause-effect inter-relationships of productivity and ecotoxicity

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