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Investigating the Sources of Microbial Pollution of a PermanentlyClosed Beach

The objective of this project was to examine sources of E. coli   originating on, or near, a beach area and the contribution from   these sources on E. coli levels at the beach. A microbiological   survey of Ashfield Township Park Beach on Lake Huron was conducted  from May to November, 2  5. E. coli numbers frequently exceeded the   Provincial Water Quality Objective (PWQO) of 1   cfu/1   ml of water   in the near shore lake water despite a lack of rainfall and minimal   input from Eighteen Mile Creek. We also found high E. coli numbers   (in the range of 1 3 cfu/g) associated with algae (Cladophora and  Chara species) collected in both the river and the lake. These   observations suggest a beneficial association between E. coli and   algae, and this may allow E. coli to survive and multiply on the   surface of the algae. We also observed high E. coli concentration in   a narrow section of foreshore sand and interstitial water on the   beach. Levels of E. coli in foreshore material were elevated at a   site adjacent to an algal mat in the lake, and were highest at sites   inhabited by shorebirds.     A microbial source tracking (MST) library is being generated to   differentiate contributions from fecal sources to the contamination   observed on Ashfield Township Park Beach. Repetitive element (REP)-  PCR fingerprints are being generated for each fecal isolate.   Isolates from algae will also be analyzed to determine the potential   for algae to serve as a source, rather than a sink for E. coli. We   will examine if these environmentally-adapted strains persist, or if   they are constantly regenerated by fresh fecal input.    (microbial source tracking); (MST); (bacterial source tracking);   (BST); (E. coli); (Escherichia coli); (Lake Huron); (algal blooms);   (algae); (Cladophora); (Chara); (polymerase chain reaction); (PCR);   (repetitive element); (REP); (REP-PCR)

Status
In progress
Type
Project
Start Date
End Date
Scope of Study
Field Investigation
Laboratory Investigation
Scale of Phenomena
Landscape
Land Use and Habitat
Shoreline
Lake Basin Connecting Channels
Lake Huron
State Province
Ontario
Region Being Monitored
Benthos
Near and OpenShore Waters
Nearshore Terrestrial
Resource Being Monitored
Bottom Sediment
Human Health
Societal/Surface Water
Suspended Sediments
Beneficial Use Impairment Assessments
Beach Closings
Eutrophication or Undesirable Algae
Annex Numbers
Pollution from Contaminated Groundwater
Pollution from Non-Point Sources
Remedial Action Plans and Lakewide Management Plans
Research & Development
Annex 17
Mass transfer of pollutants between GL Basin Ecosystem components
General
Monitoring
Annex
  • Annex Numbers
    Annex Numbers
    Pollution from Contaminated Groundwater
    Pollution from Non-Point Sources
    Remedial Action Plans and Lakewide Management Plans
    Research & Development
  • Annex 17
    Annex 17
    Mass transfer of pollutants between GL Basin Ecosystem components

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