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Assessment of contaminated sediments using a rainbow trout (Oncorhynchus mykiss) as an embryonic exposure assay

Rainbow trout (Oncorhynchus mykiss) have been used extensively as a toxicological model for assessing water quality due to low husbandry costs, sensitivity to pollutants and easily quantified physiological responses to contaminants. Due to inadequacies of LD5 values (dose response that results in 5 % lethality), use of early life stage (ELS) toxicity analysis has increased as a tool to characterize risk in aquatic ecosystems. However, no published studies were found addressing the use of rainbow trout embryos to evaluate toxicity associated with direct contact with sediments. This project hypothesizes that sub-lethal concentrations of organic contaminants associated with sediments will yield quantifiable health impacts using a variety of indices. Two classes of chemicals, polycyclic aromatic hydrocarbons (PAHs) and polybrominated diphenyl ethers (PDBEs), will be used separately and together to determine synergistic effects. Survival, fluctuating asymmetry, sex reversal, vitellogenin production and embryonic uptake will be examined using all-male clonal lines. This novel approach will provide insight into the toxicological and physiological effects of these compounds, as well as assess the feasibility of using this strategy as an alternative bioassay to determine sediment toxicity. Corresponding examination of sorption behavior of the organic compounds will provide further insight into the bioavailability of sediment-bound pollutants. (polycyclic aromatic hydrocarbons); (polycyclic aromatic hydrocarbon); (PAH's); (PAH); (PAHs); (polybrominated diphenyl ethers); (polybrominated diphenyl ether); (PBDE's); (PBDE); (PBDEs); (toxic organic); (toxic organics); (Oncorhynchus mykiss); (rainbow trout); (chemical of concern); (chemicals of concern); (fish consumption); (restriction on fish consumption); (restrictions on fish consumption)

(Matching Funds) $48,077 USD (Estimates)

Status
In progress
Type
Project
Start Date
End Date
Researchers
Jeffrey L. UllmanPrincipal Investigator
Associated with 1 projects
Agencies
U.S. Geological Survey $ 24,000.00USDEstimates

Funding 13 projects for a total of $538,321.00
Scope of Study
Laboratory Investigation
Scale of Phenomena
Biochemical
Organism
Physical/Chemical
Impact of Pollutants
Toxic Organics
Resource Management
Fisheries
Resource Being Monitored
Bottom Sediment
Fisheries
Habitat
Suspended Sediments
Beneficial Use Impairment Assessments
Compliance with Environmental Quality Objectives
Fish Tumours or Other Deformities
Loss of Fish and Wildlife Habitat
Restrictions of Fish and Wildlife Consumption
Tainting of Fish and Wildlife Populations
Annex Numbers
Persistent Toxic Substances
Research & Development
Specific Objectives for toxic substances and effluents
Annex 17
Action levels for multi-media exposures and interactive effects of chemicals
Ecotoxicity and toxicity effects of pollutants related to developing Annex 1 specific objectives
Relationship of contaminated sediments on ecosystem health
General
Monitoring
Annex
  • Annex Numbers
    Annex Numbers
    Persistent Toxic Substances
    Research & Development
    Specific Objectives for toxic substances and effluents
  • Annex 17
    Annex 17
    Action levels for multi-media exposures and interactive effects of chemicals
    Ecotoxicity and toxicity effects of pollutants related to developing Annex 1 specific objectives
    Relationship of contaminated sediments on ecosystem health

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Great Lakes - St. Lawrence Region.